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KMID : 0613820120220040538
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Journal of Life Science
2012 Volume.22 No. 4 p.538 ~ p.544
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Anti-Proliferative Effect of Ethanol on Normal and Cancer Cells
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Oh Myung-Ju
Kim Ji-Hyun
Park Su-Hyun
Jeong Young-Hwa
Wang Kun
Cho Byung-Wook
Jhun Byung-H.
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Abstract
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Ethanol is known as being carcinogenic to humans. In addition, the anti-proliferative effects of ethanol have been described for a variety of tissues and cells. In this study, we investigated the anti-proliferative effects of ethanol on various cancer cells, particularly on oncogenic ras-transformed or-injected cells. Ethanol treatment inhibited the cell proliferation of normal control cells, but did not suppress the proliferation of various cancer cells and oncogenic ras$ -transformed cells. Furthermore, ethanol treatment did not interfere with DNA synthesis, which was induced by microinjecting the oncogenic H-Ras^{V12} protein. The anti-proliferative effect of ethanol was rescued by antioxidants, such as N-acetylcysteine and 4-methlpyrazole. These results suggest that ethanol cytotoxicity is exerted through free radical formation, and that the anti-proliferative action site of ethanol cytotoxicity either lies upstream, or is independent of Ras.
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KEYWORD
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Ethanol, cell proliferation, Ras, microinjection, antioxidant
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